By Tiffany Carey and Courtney Maloney
One of the many signs of Spring is the United States’ report
on pollen counts across the country. These pollen counts are essential, due to
the 35 million Americans who get hay fever every year from pollen. In our
project, we investigated whether allergenic pollen concentrations from three
ecotypes of common ragweed (Ambrosia
artemisiifolia) produce more pollen in response to rising CO2 concentrations.
Our objective was to test for differences in pollen production by ecotypes from
these climatically distinct parts of New England. In order to predict when and
where pollen allergies are most likely to increase in response to climate
change, we have to determine its impact in different places.
We investigated two factors of growth and production. We
analyzed the amount of pollen produced by each ecotype, and in each of the
three CO2 concentrations. To do this we created a stratified random
subset of approximately 90 plans out of the full experimental design. The
pollen was collected and frozen from the three to five flowering spikes per
plant by covering the spikes with polyethelene bags at the time of flowering
until pollen was completely released and then placed in a sub-80C freezer.
These plants were kept in a lab at the University of Massachusetts - Amherst
(UMASS - Amherst) where we spent most of our summer. Including our mentor Kristina Stinson, the team for this project
consisted of 9 people from both Harvard Forest and UMASS’ aerobiology lab.
We also helped with the fieldwork component of the project.
Even though this portion of the project was not a part of our summer’s
analysis, we helped gather field data to determine the phenology of the ragweed
plants in 3 temperature gradients across Massachusetts. We went to various
demography plots that were in cool, warm and hot gradients and counted the
number of individual ragweed plants and identify if they are flowering and/or
releasing pollen.
Predicting how increased CO2 affects ragweed growth and pollen output and achieving greater understanding of how different local ecotypes respond to such changes, will better inform decisions regarding ragweed and allergenic plant policy and management. The importance of these results will only increase over the next several decades, as climate change increases the quantity and allergenicity of pollen in certain area via rises in CO2 concentrations and temperatures.
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